Identifying Micro-Organisms

AIM: to practice using virtually sterile techniques, to understand a range of micro-organisms demonstrate in air macroscopically and to psychoanalyze a range of micro- organisms found in food and irrigate supply macroscopically APPARATUS:         Incubator          stereotypical nutritious agar plates 1.         3 body of water ensamplings 2.         1 air warning 3.         3 food samples          etna burner         Sterile pipettes          inform loops         Sticky tape          scratch tape         Water samples(various)         Yoghurt         Inoculating molest/ probe          sick from fruit, vegetables or bread         methylated strong drink RISK ASSESMENT: 1.         Glassw argon - trick ca recitation cuts if handled incorrectly 2.         Bunsen burner - seat cause burns if non on caoutchouc brand 3.         methylated invigorate - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.          plow with care, do not conk alone glassware go about the edge of the bench 2.         take care, do not break flame unattended keep combustible liquid i.e. Methylated spirits away 3.         Keep Methylated spirits away from flames. Wear gloves when discourse it 4.         Wear gloves, lab covering and safety glasses. All utilise equipment should be placed in disinfectant.

Dispose of cultures subsequently use, in an autoclave. Wash hands onward leaving lab. Never break agar plate afterwards setting up METHOD: 1.         Sterilise the terrace area by draw alcohol on to it 2.          draw in 8 sterile nutritious agar plates 3.         A sample of fresh milk was obtained 4.         leave one plate aside, au hencetic and taped as the control 5.          use a sterile inoculating loop, a small sample of the fresh milk was streaky onto the plates. This plate was then loaded with sticky tape and labeled as fresh milk 6.          act 3 and 4 were then repeated with tap water, distilled water, set about tank water, fresh milk, one-time(a) milk, If you want to get a full essay, order it on our website: Ordercustompaper.com

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